《植物生理学报》 2010, 46(12): 1247-1250

叠氮化钠诱变玉扇愈伤组织的研究

吴正景^1,*, 张菊平¹, 时灿辉¹, 巩振辉²

1河南科技大学林学院, 河南洛阳471003; 2西北农林科技大学园艺学院, 陕西杨凌712100

通信作者：吴正景；Tel: 2010-09-15

摘　要：

在含不同浓度叠氮化钠(0、0.01、0.05、0.1、0.5 和1.0 mmol·L-1)的诱导培养基上诱导玉扇愈伤组织发生变异。结果表明, 叠氮化钠对玉扇愈伤组织的半致死浓度为0.1~0.5 mmol·L-1, 0.1 mmol·L-1 有利于保证一定量的有效变异率和再生率。再生芽多发生叶片失绿、畸形和叶序改变, 其中较多的叶序变异, 有望为研究叶序决定提供材料。

关键词：玉扇; 叠氮化钠; 诱变; 叶序

收稿：2010-09-15   修定：2010-10-15

资助：陕西省“13115”科技创新工程重大科技专项(2007ZDKG-05)和河南科技大学博士科研基金(09001216)。

Study of Sodium Azide Inducing Effect on Callus of Haworthia Truncata var. truncata

WU Zheng-Jing^1,*, ZHANG Ju-Ping¹, SHI Can-Hui¹, GONG Zhen-Hui²

¹College of Forestry, Henan University of Science and Technology, Luoyang, Henan 471003, China;²College of Horticulture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China

Corresponding author: WU Zheng-Jing; Tel: 2010-09-15

Abstract:

In this study sodium azide (0, 0.01, 0.05, 0.1, 0.5, 1.0 mmol·L^-1 in tissue culture medium) was used to induce callus of Haworthia truncata. The results showed that the half-death dosage of sodium azide to Haworthra truncata callus was between 0.1 and 0.5 mmol·L^-1, but 0.1 mmol·L^-1 sodium azide was able to insure some useful variations that mostly were leaf color, shape and phyllotaxis variation, among all the mutations, phyllotaxis variation could provide material to study leaf arrangement.

Key words: Haworthia truncata; sodium azide; inducement; phyllotaxis